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KMID : 0545120070170030420
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 3 p.420 ~ p.427
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Temperature Effects on Korean Entomopathogenic Nematodes, Steinernema glaseri and S. longicaudum, and their Symbiotic Bacteria
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Hang Dao-Thi
Choo Ho-Yul
Lee Dong-Woon
Lee Sang-Myeong
Kaya Harry K.
Park Chung-Gyoo
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Abstract
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We investigated the temperature effects on the virulence, development, reproduction, and motility of two Korean isolates of entomopathogenic nematodes, Steinernema glaseri Dongrae strain and S. longicaudum Nonsan strain. In addition, we studied the growth and virulence of their respective symbiotic bacterium, Xenorhabdus poinarii for S. glaseri and Xenorhabdus sp. for S. longicaudum, in an insect host at different temperatures. Insects infected with the nematode-bacterium complex or the symbiotic bacterium was placed at 13oC, 18oC, 24oC, 30oC, or 35oC in the dark and the various parameters were monitored. Both nematode species caused mortality at all temperatures tested, with higher mortalities occurring at temperatures between 24oC and 30oC. However, S. longicaudum was better adapted to cold temperatures and caused higher mortality at 18oC than S. glaseri. Both nematode species developed to adult at all temperatures, but no progeny production occurred at 13oC or 35oC. For S. glaseri, nematode progeny production was best at inocula levels above 20 infective juveniles/host at 24oC and 30oC, but for S. longicaudum, progeny production was generally better at 24oC. Steinernema glaseri showed the greatest motility at 30oC, whereas S. longicaudum showed good motility at 24oC and 30oC. Both bacterial species grew at all tested temperatures, but Xenorhabdus sp. was more virulent at low temperatures (13oC and 18oC) than X. poinarii.
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KEYWORD
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Steinernema, mutualistic bacterium, Xenorhabdus, entomopathogenic nematode
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